Research in Spectroscopy

Time resolved Fluorescence Spectroscopy:

Faculty: Dr. Renuka C G

Doctoral Students:

Mr.Anil Kumar.
Mr.Pramod A G.
Mr.Mohamed Zikriya.
Mr. Venkatesha Babu K R.

 Our study of orientational dynamics is aimed at understanding the motions of solute molecules in their solvent environment. The nature and time scale of molecular motions is changed dramatically when a molecule is placed in a solution. One of the simplest approaches for probing solute-solvent interaction is to determine how simple molecular motions are altered by the solvent. Since optical spectroscopic methods are capable of measuring reorientational dynamics on the time scale that are relevant to solute-solvent interactions, we use time-resolved spectroscopy to probe reorientational motions.

 We use fluorescence depolarization measurements to study the dynamics of simple peptides in solution. The goal of these experiments is to determine the time scale and amplitudes of internal and overall motions of peptides. An example of this work is our study of intermolecular dynamics in opioid peptides, including enkephalin. We have found that these peptides undergo a conformational change at low temperatures that leads to a freezing up of internal motion. Further experiments are planned to see what intramolecular interaction is important in this process.

state decay

depolarisation

tcspc

Techniques:

  • Fluorescence correlation spectroscopy
  • Fluorescence anisotropy spectroscopy
  • Fluorescence lifetimes and quantum yields
  • Measurements of fluorescence lifetimes
  • Phase-sensitive detection of fluorescence
  • Fluorescence polarization
  • Time dependent decays of fluorescence anisotropy
  • Effects of solvents on fluorescence emission spectra
  • Mechanisms and dynamics of solvent relaxation
  • Quenching of fluorescence
  • Protein fluorescence